Composite

Part:BBa_K2467000

Designed by: Alreem Johar   Group: iGEM17_CMUQ   (2017-10-26)
Revision as of 13:38, 26 October 2017 by Registry (Talk | contribs)

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DsbA DspB emGFP

For our improvement of part BBa_K1659211 which is DspADspB (dispersin exported to the extracellular), we amplified the DspADspB and incorporated EcoR1 and BamH1 restriction sites. The gene was ligated onto the multiple cloning site on the pRSET emgfp vector which resulted in fluorescently tagged DspADspB. This allows for easy fluorescence detection in the supernatant contributed by the exported DspADspB. Projects dealing with disruption of Biofilms can easily follow the release of the dispersin enzyme into the extracellular environment to degrade the biofilm polysaccharides

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 316
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 472


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Parameters
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