Composite
Part:BBa_K2350022:Design
Designed by: CHIA-SUI CHIANG Group: iGEM17_NYMU-Taipei (2017-10-23)
pPIGBACK-PrbcL-CrtZ
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We used fusion PCR to fuse CrtZ and PrbcL to avoid restriction site in between which might influence expression efficiency.
Source
pPIGBACK is from BBa_K2350009, and CrtZ is from Pantoea ananatis.