Composite

Part:BBa_I714040:Design

Designed by: Mingzhi Qu   Group: iGEM07_Peking   (2007-10-20)
Revision as of 03:21, 23 October 2007 by Sunnyboy (Talk | contribs) (→‎Design Notes)

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[I714030][J23066] ([F-oriT][Key2][Double Terminator])


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 278
    Illegal NheI site found at 324
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 484
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 361


Design Notes

We simply ligate F-oriT and J23066.

Source

I714030 + J23066

References