Part:BBa_K2368028

Introduction

Gα- MAPK pathway signal transduction factor

Gα plays an important role in Saccharomyces Cerevisiae CEN.PK2-1C MAPK pathway. In the yeast endogenous metabolic pathway, the STE2’s conformation changes and combines with the Gα, forcing Gα dissociates with Gβ/Gγ dimer. The initial sequence Gα is used to pass the signal to the downstream.

Fig.1 The structure of Gα

Design

Use yeast Gα fusion to GPCR protein to make signal transduction, and we obtain the part by replicating the sequence from the yeast.

Fig.2 The schematic diagram of Modified Gα

Experiment

The part is used to transduct the signal that from the sweetness receptor to the downstream. The length of sequence is 1421bp.

Fig.3 The electrophoresis of the positive result of Gα.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]