Composite

Part:BBa_K2505030

Designed by: Hazuki Hasegawa   Group: iGEM17_TokyoTech   (2017-10-17)
Revision as of 08:37, 26 October 2017 by TakumaY (Talk | contribs)

Ptet-rbs-traI (K34G)-tt

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


This part constitutively produces C8. We introduced point mutation to train gene, the productivity of C8 was improved by 100-fold. We introduced this part to E. coli then E. coli could produced enough C8 to induce transcription of human cells.


Characterization

Materials & Methods

[edit]
Categories
Parameters
None