Protein_Domain
Part:BBa_K2356001:Design
Designed by: Ralf Philipsen Group: iGEM17_TU-Eindhoven (2017-10-26)
14-3-3 tetramer with GFP
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1306
Illegal EcoRI site found at 1461
Illegal PstI site found at 521
Illegal PstI site found at 3967 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1306
Illegal EcoRI site found at 1461
Illegal NheI site found at 1642
Illegal PstI site found at 521
Illegal PstI site found at 3967 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1306
Illegal EcoRI site found at 1461
Illegal BamHI site found at 3229 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1306
Illegal EcoRI site found at 1461
Illegal PstI site found at 521
Illegal PstI site found at 3967 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1306
Illegal EcoRI site found at 1461
Illegal PstI site found at 521
Illegal PstI site found at 3967
Illegal AgeI site found at 3958 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 1009
Illegal SapI.rc site found at 2602
Design Notes
Flexible linkers were required between the the domains.
Source
The 14-3-3 protein sequence originates from tobacco plants and the DNA sequence has been optimized for expression in E. coli.