Regulatory

Part:BBa_K2247000:Design

Designed by: Jianing Han   Group: iGEM17_Tsinghua   (2017-10-23)
Revision as of 07:10, 26 October 2017 by GBY15 (Talk | contribs) (→‎Design Notes)

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pGalS promoter of S. cerevisiae


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 453
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 54
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is a Gal promoter from the yeast S. cerevisiae, modified from the endogenous Gal1 promoter. Endogenous Gal1 promoter contains upstream activating sequence (UAS), where lies the binding sites of the yeast Gal4 activator. Similar to pGal1, the expression from pGalS can be induced by Gal4 protein, with a higher strength than pGal1.

Source

Modified by Dr. Junbiao Dai's Group from Tsinghua University

References