Protein_Domain

Part:BBa_K1753001

Designed by: Yunfei Dai   Group: iGEM15_WHU-Pharm   (2015-09-16)
Revision as of 06:02, 26 October 2017 by Sinicka tanua (Talk | contribs)

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RNA polymerase omega subunit

This part contains a sequence that codes for RNA polymerase omega subunit. Expressed with other 4 subunits, this subunit can be used to assemble RNA polymerase core enzyme.

It is the smallest subunit of bacterial RNA polymerase (amino acid 91) of E. coli. During assembly of a core enzyme it attaches last and contacts with b’-subunit and only with it. This subunit has been demonstrated to be essential for the correct folding of a b’-subunit, participate in attracting an s-subunit to a core enzyme and be a target for some transcriptional factors regulating the functioning of RNA polymerase (ppGpp).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Sequence:

ATGGCACGCGTAACTGTTCAGGACGCTGTAGAGAAAATTGGTAACCGTTTTGACCTGGTACTGGTCGCCGCGCGTCGCGCTCGTCAGATGCAGGTAGGCGGAAAGGATCCGCTGGTACCGGAAGAAAACGATAAAACCACTGTAATCGCGCTGCGCGAAATCGAAGAAGGTCTGATCAACAACCAGATCCTCGACGTTCGCGAACGCCAGGAACAGCAAGAGCAGGAAGCCGCTGAATTACAAGCCGTTACCGCTATTGCTGAAGGTCGTCGTTAA


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Categories
Parameters
None