Coding

Part:BBa_K2230022

Designed by: Yi-Lun Huang & Pei-Hong Chen   Group: iGEM17_Mingdao   (2017-10-19)
Revision as of 04:11, 26 October 2017 by Ryan2829 (Talk | contribs)


STM1128/pSB1C3


Mingdaophil1026-1.jpeg

Salmonella typhimurium LT2 has two glucose-specific transporter systems, PTS system and sodium/glucose cotransporter. PTS system contains two subunits IIA encoded by crr and IIBC by ptsG which are assembled to a high-affinity active transporter. The other is a Na+/glucose cotransporter encoded by STM1128 that contributes to facilitated transport with lower glucose affinity.


Research

Based on our research, the glucose transporter of Salmonella has a lower Km compared to human small intestine, Staphylococcus and E. coli, indicating a higher efficiency for glucose uptake.

Mingdaophil1026-2.png


Cloning

The STM1128 gene was amplified from gDNA of Salmonella typhimurium and cloned onto pSB1C3. This part has been sequenced.

Mingdaophil1026-3.jpeg


Demonstration for Glucose Absorption

In order to express the genes, we chose promoter CP29 that is a strong constitutive promoter working well in both E. coli and Lactobacillus spp. The biobrick part, CP29-RBS-aeBlue (BBa_K1033280), was used and assembled with the transporter genes.

Mingdaophil1026-4.jpeg



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1255
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//awards/basic_part/winner
//chassis/prokaryote/ecoli
//chassis/prokaryote/lactococcus
//collections/probiotics/production
Parameters
None