Part:BBa_K2202000:Design
ssDNA for producing in-vivo tetrahedral structure Strand1
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 182
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 101
Illegal SapI site found at 272
Design Notes
This sequence is a modification of the parts BBa K2054001,BBa K2054002, BBa K2054003 ,BBa K2054004 , BBa K2054005 from the iGEM Hong University Team 2016 team. This year our team is produced a Pre-tetrahedron structure which folds upon binding to the Huntington's disease biomarker Hsa-miR-34b.
Shown below is the schematic of how the Pre-tetra nanostructure folds only upon binding to the target to produce a G-quadruplex and hence we expect the signal to noise ratio to improve from last year's nanostructure.
Gel Images
The gel bands of the individual oligonucleotides allow for the estimation and verification of the sizes of the oligonucleotides by comparison with the DNA ladder. The formation of the 3-dimensional structure from the 2-dimensional DNA nanostructure in the presence of the specific target can also be clearly observed from the above gel image as a prominent shift from the gel band in lane 9 to the gel band in lane 10 can be distinctly seen.
Elbaz, Johann, Peng Yin, and Christopher A. Voigt. "Genetic encoding of DNA nanostructures and their self-assembly in living bacteria." Nature Communications 7 (2016).
Source
This Part was generated using a DNA nanotechnology software called Tiamat. This is a computationally designed sequence.