Plasmid

Part:BBa_I739203

Designed by: Stefan Luzi   Group: iGEM07_ETHZ   (2007-10-21)
Revision as of 20:32, 22 October 2007 by Stefan Luzi (Talk | contribs)

No part name specified with partinfo tag.

Plasmid pCK01MCS1 is based on pCK01 [1], a low-copy cloning vector that confers chloramphenicol resistance (CmR) and harbours an oriV.
Pck01 Andy.jpg

In order to make this plasmid compatible with the BioBrick Standard assembly process, a MCS harbouring a SpeI site was introduced by PCR. This MCS is the only difference between pCKMCS1 and pCK01:
PCK01-2.jpg


Purpose

This plasmid was designed for the [http://parts.mit.edu/igem07/index.php/ETHZ ETHZ iGEM 2007 project] and contains...


References

[1] Fernández S, de Lorenzo V, Pérez-Martín J "Activation of the transcriptional regulator XylR of Pseudomonas putida by release of repression between functional domains", Mol Microbiol. 16(2):205-13, 2000


Sequence and Features No part name specified with partinfo tag.


[edit]
Categories
Parameters
chassis
copies15-20
mcsBioBrick
originpMB1
resistanceT