Plasmid_Backbone

Part:BBa_K2491030:Experience

Designed by: Philippe Hansen-Estruch   Group: iGEM17_CCA_San_Diego   (2017-10-15)
Revision as of 21:51, 25 October 2017 by WokeXD (Talk | contribs) (→‎Applications of BBa_K2491030)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K2491030

Clone verification. The following primers annealing in the TRFA region of the RK2 backbone vector and the kanamycin region were used to verify the construct by amplification and sequencing of the backbone: TRFA_F: 5’-CCGACGGATGTTCGACTATTT-3’, TRFA_R: 5’-AGTAAAGCGCTGGCTGAA-3’, KM_F: 5’-AAAGCCGTTTCTGTAATGAAGG-3’, and KM_R: 5’-CCTGTTGAACAAGTCTGGAAAG-3’. The data showed that the 2 regions were present. In addition, cultures containing the RK2 backbone harboring the RFP gene [biobrick part BBa_K2491030 containing biobrick part BBa_J04450] showed a red pellet (right vial) compared to a control (left vial) without the RFP gene.

(Include Picture Here)

User Reviews

UNIQ560db6c008749e11-partinfo-00000000-QINU UNIQ560db6c008749e11-partinfo-00000001-QINU