Plasmid

Part:BBa_I739203

Designed by: Stefan Luzi   Group: iGEM07_ETHZ   (2007-10-21)
Revision as of 20:17, 22 October 2007 by Stefan Luzi (Talk | contribs)

No part name specified with partinfo tag.

Plasmid pCK01MS1 is based on pCK01 [1], a low-copy cloning vector that confers chloramphenicol resistance (CmR) and harbours an oriV.
Pck01 Andy.jpg

In contrast to the original cloning vector, we removed an intrinsic SpeI site by PCR and also introduced the following MCS:
PCK01-2.jpg



References

[1] Fernández S, de Lorenzo V, Pérez-Martín J "Activation of the transcriptional regulator XylR of Pseudomonas putida by release of repression between functional domains", Mol Microbiol. 16(2):205-13, 2000


Sequence and Features No part name specified with partinfo tag.


[edit]
Categories
Parameters
chassis
copies15-20
mcsBioBrick
originpMB1
resistanceT