Coding

Part:BBa_K2235008

Designed by: Shanlin Tong, Shivashree Dhanaraj, Sina Amoor Pour and Gilai Nachmann   Group: iGEM17_Stockholm   (2017-10-11)
Revision as of 20:05, 25 October 2017 by Tongshl (Talk | contribs) (→‎Usage and Biology)


Endo beta galactosidase

Usage and Biology

The basic part consists of the endo-beta galactosidase enzyme coding sequence with a His-tag C-terminally attached. This enzyme has been shown to release saccharide chains from glycans expressed in gastric mucus. It performs this action by hydrolysing the bonds next to galactose saccharides in the polysaccharide chains of mucin. The sequence originates fro=m the species Clostridium Perfringens.


Figure x: mechanism of endo-beta galactosidase enzyme

You can find more details about endo-beta galactosidase in its composite part BBa_K2235010.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 174
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/ecoli
//collections/probiotics/production
Parameters
None