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Part:BBa_K2235009:Experience

Designed by: Shivashree Dhanaraj, Shanlin Tong, Gilai Nachmann and Sina Amoor pour   Group: iGEM17_Stockholm   (2017-10-11)
Revision as of 18:36, 25 October 2017 by Shiva (Talk | contribs) (→‎Molecular cloning)


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Molecular cloning

Ligation of sialidase composite insert into pSB1C3

The gblock containing T7 promoter-RBS-sialidase was ligated into the Chloramphenicol plasmid backbone (pSB1C3). Preliminary confirmation of cloning was done by double digest Ecor1 and Pst1. Figure 1 represents the double digestion result: bands were visible at ~1600 bp and ~2000 bp which represents the insert and plasmid backbone respectively.

Figure 1

From left to right: M DNA ladder, next 2 lanes are plasmids digested with Ecor1 and Pst1.

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