Coding

Part:BBa_K2433002

Designed by: Tamar Av-Shalom   Group: iGEM17_British_Columbia   (2017-10-25)
Revision as of 18:08, 25 October 2017 by Registry (Talk | contribs)

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ietAS

ietAS is composite part made up of ietA an ietS the genes encoding the toxin antitoxin system ietAS native to tumor-inducing (Ti) plasmids of Agrobacterium tumefaciens. This part comes from the nopaline type Ti plasmid pTiC58. In combination with the toxin gene, this part has been shown in literature to be important in reducing the transconjugant efficiency for different incoming Ti plasmids as well as contributing to the stability of plasmids harboring this construct.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 737
    Illegal BamHI site found at 1758
    Illegal XhoI site found at 659
    Illegal XhoI site found at 989
    Illegal XhoI site found at 3353
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2663
    Illegal NgoMIV site found at 3547
    Illegal AgeI site found at 3704
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 49
    Illegal BsaI.rc site found at 836
    Illegal BsaI.rc site found at 1838
    Illegal BsaI.rc site found at 3023
    Illegal BsaI.rc site found at 3305
    Illegal SapI.rc site found at 373


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Categories
Parameters
None