Coding

Part:BBa_K2433001

Designed by: Tamar Av-Shalom   Group: iGEM17_British_Columbia   (2017-10-25)
Revision as of 18:06, 25 October 2017 by T.avshalom (Talk | contribs)


ietS

ietS is the gene encoding the toxin in the toxin antitoxin system ietAS native to tumor-inducing (Ti) plasmids of Agrobacterium tumefaciens. This part comes from the nopaline type Ti plasmid pTiC58. In combination with the toxin gene, this part has been shown in literature to be important in reducing the transconjugant efficiency for different incoming Ti plasmids as well as contributing to the stability of plasmids harboring this construct. Data base searches have shown that IetA is similar in structure to serine proteases.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 425
    Illegal XhoI site found at 2020
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1330
    Illegal NgoMIV site found at 2214
    Illegal AgeI site found at 2371
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 505
    Illegal BsaI.rc site found at 1690
    Illegal BsaI.rc site found at 1972


[edit]
Categories
Parameters
None