Part:BBa_K2368028

Introduction

BBa_K2368028 ort Not understood

Gα plays an important role in Saccharomyces Cerevisiae CEN.PK2-1C MAPK pathway. In the yeast endogenous metabolic pathway, the STE2’s conformation changes and combines with the Gα, forcing Gα dissociates with Gβ/Gγ dimer. The initial sequence Gα is used to pass the signal to the downstream.

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Fig.1 The structure of Gα

Design

Use yeast Gα fusion to GPCR protein to make signal transduction, and we obtain the part by replicating the sequence from the yeast.

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Fig.2 The schematic diagram of Modified Gα

Experiment

The part is used to transduct the signal that from the sweetness receptor to the downstream. The length of sequence is 1421bp.

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Fig.3 The electrophoresis of the positive result of Gα.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]