Composite
Part:BBa_K2235009:Experience
Designed by: Shivashree Dhanaraj, Shanlin Tong, Gilai Nachmann and Sina Amoor pour Group: iGEM17_Stockholm (2017-10-11)
Revision as of 17:48, 25 October 2017 by Shiva (Talk | contribs) (→Characterizations of BBa_K2235009)
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Characterizations of BBa_K2235009
Molecular cloning
Ligation of sialidase composite insert into pSB1C3 The gblock containing T7 promoter-RBS-sialidase was ligated into the Chloramphenicol plasmid backbone (pSB1C3). Preliminary confirmation of cloning was done by double digest Ecor1 and Pst1. Figure 1 represents the diggestion result: bands were visible at ~1600 bp and ~2000 bp which represents the insert and plasmid backbone respectively.
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