Part:BBa_K2208001
A coding sequence of tPep-Lamp2b fusion protein and position it outside the membrane.
tPep is a white fat tissue targeting peptide. To enhance the accuracy of siRNA delivery system and improve targeting index of drugs, tPep was displayed on the surface of the exosomes containing our previously designed siRNAs, allowing us to target recipient cells in vivo efficiently. The adipocyte targeting capability of exosomes was conferred by engineering the human embryonic kidney 293 (HEK293) cells to express lysosome-associated membrane glycoprotein 2b (Lamp2b), a well-characterized exosomal membrane protein, fused with tPep (CKGGRAKDC) for integrin. Lamp-2b is a protein found specifically abundant in exosomal membranes. So we connect tPep with Lamp2b by a glycine-linker, and promote the expression using cmv promoter. In that way, the tPep exosomes (tPep-Exos) are endowed with site-specific identification ability and were purified from cell culture supernatants and loaded with siRNA.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1276
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 199
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