Coding
Part:BBa_K2208001:Design
Designed by: iGEM17_NJU-China Group: iGEM17_NJU-China (2017-10-25)
A coding sequence of tPep-Lamp2b fusion protein and position it outside the membrane.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1276
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 199
Design Notes
In our design, the tPep is inserted between the signal peptide and the N-end of the Lamp2b so that the fusion protein will fold and work normally. And we make sure the tPep will show up at the surface of exosomes.
Source
Artificially designed.