Part:BBa_I742123

Multi-host vector pTG262 converted to BioBrick vector

biobricked version of pTG262

Plasmid has a multi host replication origin and is competant in range of gram positive and gram negative bacteria.

More information about pTG262 may be found on the [http://parts.mit.edu/igem07/index.php/Edinburgh/Yoghurt/Design#Gene_Expression_In_Lactobacillus Edinburgh Yoghurt Wiki]

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 5543
  Illegal NheI site found at 694
  Illegal SpeI site found at 2
  Illegal PstI site found at 16
  Illegal NotI site found at 9
  Illegal NotI site found at 5549
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 5543
  Illegal BglII site found at 3754
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal prefix found at 5543
  Illegal suffix found at 2
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal prefix found at 5543
  Plasmid lacks a suffix.
  Illegal XbaI site found at 5558
  Illegal SpeI site found at 2
  Illegal PstI site found at 16
  Illegal AgeI site found at 3365
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal SapI.rc site found at 3578