Composite

Part:BBa_K2306010

Designed by: Guillermo Serena Ruiz   Group: iGEM17_TUDelft   (2017-10-04)
Revision as of 14:24, 25 October 2017 by Gserenaruiz (Talk | contribs)


Cytosolic-abundant heat soluble protein 106094 "CAHS 106094" (T7 promoter, RBS and double terminator

This Biobrick features the gene sequence that encodes for the production of the tardigrade intrinsically disordered protein (TDP) "CAHS 106094". This is one of the several heat soluble proteins found in the tardigrades capable of protecting other fragile biological parts upon desiccation by generating a crystal matrix. Therefore, this tardigrade protein can be combined with other perishable biological material (e.g. proteins) to increase its stability after dehydration. The sequence encoding for the gene is preceded by a T7 promoter (BBa_I712074) and an RBS, which allows inducible overexpression of the protein with IPTG; and followed by a double terminator consisting of a rrnB T1 terminator and T7Te terminator (BBa_B0015).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None