Composite

Part:BBa_K2342006

Designed by: Ushanandini Mohanraj   Group: iGEM17_Aalto-Helsinki   (2017-10-25)
Revision as of 12:53, 25 October 2017 by Registry (Talk | contribs)

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DCD1L peptide linked to sumo fusion peptide with His6x tag

This part comprises of a Dermcidin derived antimicrobial peptide DCD-1L. For the efficient production and purification system, it is combined with a SUMO fusion protein Smt3 and his tag. Using this part it is possible to produce the DCD-1l in E.coli without killing the host bacteria. This can then be purified using his tag affinity chromatography. Then the His Smt3 tag could be cleaved using the Ulp1 protease enzyme obtaining a free DCD-1L protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 127
    Illegal BglII site found at 410
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None