Part:BBa_K2282005
BBa_K592009 (AmilCP) consitutive expression system.
AmilCP is expressed thanks to the Anderson constitutive promoter and translation is initiated at the ribosome binding site. AmilCP is a chromoprotein that has already been successfully used by several iGEM teams and leads to a strong color visible to the naked eye (The protein has an absorbance maximum at 588 nm) but also a temperature-dependent expression when used with a weak promoter. The double terminator enables robust termination of transcription.
Usage and Biology
AmilCP is expressed thanks to the Anderson constitutive promoter and translation is initiated at the ribosome binding site. AmilCP is a chromoprotein that has already been successfully used by several iGEM teams and leads to a strong color visible to the naked eye (The protein has an absorbance maximum at 588 nm) but also a temperature-dependent expression when used with a weak promoter. The double terminator enables robust termination of transcription.
Source of this part
The genomic sequence has been found on the IGEM registry of standard biological parts and the part has been assembled.
Design consideration
None.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
| None |