Part:BBa_I746105

GFP with agr P2 promoter

This is composed of a promoter and a GFP reporter gene. This can produce green fluorescence and in theory the strength of the green flourence is much stronger when there is phosphorylated AgrA in the cell. This is used to test the basal activity of agr P2 promoter.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 769
  

Contribution：TMMU-China 2017

Author：Yizhen Xu

Summary：In this contribution, we characterized this part in S.aureus.

Characterization in S.aureus

To characterize whether this P2-GFP part can function also in the Gram-positive strain, we test this composite part directly in S.aureus. The P2-GFP composite part was cut from the pSB1C3-P2-GFP plasmid, then it was ligated into the shuttle vector pLI50. After that the pLI50-P2-GFP was transformed into the S.aureus strain RN4220. We found that the RN4220::pLI50-P2-GFP strain show strong green fluorescence compared to the RN4220::pLI50 strain. This data suggest that the P2-GFP composite can be functional when the Agr system is present.

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