Part:BBa_K2282002

5' Untranslated Transcribed Region of the CspA gene

Usage and Biology

The 5’ Untranslated Transcribed Region of the CspA gene is an unusually long sequence that is common to all the cold-shock protein genes (with slight variations). It is believed to be one of the master regulators of the cold-induced translation of cold-shock mRNA at low temperatures. The 5’ UTR contains a regulatory sequence called “Cold Box”​ that plays an important role in the stabilisation of the mRNA at low temperature (ATTAAA) (Mitta et al, 1997). The structure of this 5’UTR is extremely unstable at high temperature, as it is believed to be cut down by a RNAse, leading to a 12 sec mRNA stability at 37°C against 20 minutes at 15°C, temperature at which the RNAse is no longer active (Barria et al. 2013).

Source of this part

The 5’UTR sequence has been taken from the NCBI GenBank on the Cold-shock protein A gene sequence. Mitta et al, 1997 clearly indicated its position so we just had to take the sequence from here.

Design consideration

Please keep in mind that this part has already a RBS sequence located on the 5’UTR, which is the Shine-Dalgarno sequence (YAMANAKA et al, 1999) : 5’AAGG 3’

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]