Composite

Part:BBa_K2350013

Designed by: Priscilla Lee Ann Hweek   Group: iGEM17_NYMU-Taipei   (2017-10-21)
Revision as of 11:50, 25 October 2017 by Hibarihweek (Talk | contribs)

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PrbcL-CrtW-CrtZ-rrnB

This is a combination sequence that contains Crt-W. Crt-Z, a special design constitutive promoter, operon and a terminator. As the research of the pigment synthesis pathway, we can simply know that Crt-W and Crt-Z both need to be involved in this pathway to produce astaxanthin. And with a constitutive promoter, we can ensure that the gene will keep transcript and translate in-vivo so that the synthesis pathway through astaxanthin will keep inducing.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 420
    Illegal BglII site found at 1160
    Illegal BamHI site found at 1814
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2016


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Categories
Parameters
None