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Part:BBa_K2332053

Designed by: Paola Handal   Group: iGEM17_UCL   (2017-10-21)
Revision as of 11:38, 25 October 2017 by Paola handal (Talk | contribs)


Blue light inducible expression of GFP-SpyTag (Pblind GFP-SpyTag)

We designed our GFP-SpyTag Biobrick to test bacterial cell adhesion through fluorescence microscopy. This part produces the GFP-SpyTag fusion protein, which we experimentally used in combination with our Intimin-SpyCatcher Biobrick (see experiment).

To use the GFP-Spytag, one needs to transform E. Coli cells with our Biobrick and allow the cells to produce the fusion protein. Then, cells are lysed and the protein purified to be used in the experiment.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 723


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