Composite

Part:BBa_K2332053

Designed by: Paola Handal   Group: iGEM17_UCL   (2017-10-21)
Revision as of 11:30, 25 October 2017 by Paola handal (Talk | contribs)


No part name specified with partinfo tag.

We designed our GFP-SpyTag Biobrick to test bacterial cell adhesion through fluorescence microscopy. This part produces the GFP-SpyTag fusion protein, which we experimentally used in combination with our Intimin-SpyCatcher Biobrick (see experiment).

To use the GFP-Spytag, one needs to transform E. Coli cells with our Biobrick and allow the cells to produce the fusion protein. Then, cells are lysed and the protein purified to be used in the experiment.

Sequence and Features No part name specified with partinfo tag.


[edit]
Categories
Parameters
None