Composite

Part:BBa_K2332000

Designed by: Paola Handal Marquez   Group: iGEM17_UCL   (2017-09-03)
Revision as of 11:29, 25 October 2017 by Paola handal (Talk | contribs)


No part name specified with partinfo tag.

We designed our GFP-SpyTag Biobrick to test bacterial cell adhesion through fluorescence microscopy. This part produces the GFP-SpyTag fusion protein constitutively, which can then be cell extracted and purified to use in combination with cells expressing our Intimin'-SpyCatcher construct variants. SpyTag (13 amino acids) and SpyCatcher (138 amino acids, 15 kDa) are protein binding partners that originate from CnaB2 (immunoglobulin-llike collagen adhesin domain) of the FbaB protein, found in the invasive strains of S. pyogenes.

Figure 1: E. coli cells constitutively expressing GFP-SpyTag

Sequence and Features No part name specified with partinfo tag.


[edit]
Categories
Parameters
None