Coding

Part:BBa_K2381010

Designed by: Wentao Yu   Group: iGEM17_HZAU-China   (2017-10-20)
Revision as of 09:58, 25 October 2017 by Johnson Huang (Talk | contribs)


Cod1+ rigid linker+pMagFast

In this part, the light-induced dimerization protein,pMag[1], is fused with the N fragment of split dCas9 through rigid linker.
The alpha-helix structure of the rigid linker renders more cooperativity between split dCas9 and dimerization protein.
Its protein sequence is A(EAAAK)3A, in which the flanking Ala guarantees a flexibility conjunction, which can decrease the effect on the function of protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1099
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2277
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None