Regulatory

Part:BBa_K2447013

Designed by: Chee Wai Kit (david)   Group: iGEM17_NUS_Singapore   (2017-10-05)
Revision as of 09:15, 25 October 2017 by David135 (Talk | contribs)


Temperature-sensitive repressible system

Tlpa36 proteins is constitutively produced, and depending on the temperature (if below 36 degree), it will form Tlpa36 dimers and capable of repressing the downstream promoter pTlpa36; providing steric hindrance to prevent RNA polymerase to bind and elicit downstream expression.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 526
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 811
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1114
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/ecoli
//collections/probiotics/control
Parameters
chassisE.coli (beta 10)