Regulatory
Part:BBa_K2447013
Designed by: Chee Wai Kit (david) Group: iGEM17_NUS_Singapore (2017-10-05)
Temperature-sensitive repressible system
Tlpa36 proteins is constitutively produced, and depending on the temperature (if below 36 degree), it will form Tlpa36 dimers and capable of repressing the downstream promoter tlpa36; providing steric hindrance to prevent RNA polymerase to bind and elicit downstream expression.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 526
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 811
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1114
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//chassis/prokaryote/ecoli
//collections/probiotics/control
//collections/probiotics/control
chassis | E.coli (beta 10) |