![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K2244010
Designed by: Chen Hong Group: iGEM17_SSTi-SZGD (2017-10-25)
ColE promoter +supernova gene +Lev1 gene
The device is a functional plasmid containing a suicide gene supernova(BBa_K1491017), which is a mutant form of KillerRed(BBa_K1184000), the first genetically-encoded photosensitizer. Upon illumination, reactive oxygen species (ROS) are generated to induce cell apoptosis , We incorporated into our lightOFF system (BBa_K2244009) so that supernova can be induced fully in darkness and start secreting ROS upon light irradiation to promote cell death.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1045
Illegal NheI site found at 1068 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 213
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1783
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |