Composite

Part:BBa_K2244010

Designed by: Chen Hong   Group: iGEM17_SSTi-SZGD   (2017-10-25)
Revision as of 08:41, 25 October 2017 by Registry (Talk | contribs)

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ColE promoter +supernova gene +Lev1 gene

The device is a functional plasmid containing a suicide gene supernova(BBa_K1491017), which is a mutant form of KillerRed(BBa_K1184000), the first genetically-encoded photosensitizer. Upon illumination, reactive oxygen species (ROS) are generated to induce cell apoptosis , We incorporated into our lightOFF system (BBa_K2244009) so that supernova can be induced fully in darkness and start secreting ROS upon light irradiation to promote cell death.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1045
    Illegal NheI site found at 1068
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 213
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1783
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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