Composite

Part:BBa_K2244009

Designed by: Chen Hong   Group: iGEM17_SSTi-SZGD   (2017-10-25)
Revision as of 08:32, 25 October 2017 by Registry (Talk | contribs)

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ColE promoter+mCherry gene+T1 terminator+Constitutive promoter+Lev1 gene+T1terminator

The device is a functional plasmid containing a light repressor LEV1 (BBa_K2244005), which undergoes conformational change upon light irradiation and dimerized to bind regulatory sequence on ColE promoter (BBa_K2244006), thus inhibits target gene expression. While in darkness, dimerization does not occur thus gene expression proceeds. This device is a light repressing system with high induction efficiency and low leakage. mCherry (BBa_K2244008) is reporter gene used in this system to prove its function as well as help to characterize it. mCherry gene can be replaced by any gene sequence in light-regulated studies

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1015
    Illegal NheI site found at 1038
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 845
    Illegal AgeI site found at 1753
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None