Measurement

Part:BBa_I732913

Designed by: Ma Rui   Group: iGEM07_USTC   (2007-10-17)
Revision as of 14:31, 22 October 2007 by LiuZiqing (Talk | contribs)

[aTC] -> RFP

A chemical signal(aTc concentration) to red fluorescence intensity(RFP,red fluorescence protein) converter. When [http://openwetware.org/wiki/ATc aTc (anhydrotetracycline)] is present, the repression of Ptet (Part:BBa_R0040) by constitutively expressed tetR(Part:BBa_I732080)(regulated by constitutive promoter PlacIQ(Part:BBa_I14032)) is relieved. Thus the mRFP (Part:BBa_E1010) coding sequence downstream of Ptet could be transcribed. It is used to test the function of Part:BBa_I732086.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1469
    Illegal AgeI site found at 1581
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None