Coding

Part:BBa_K2381000:Design

Designed by: Wenqi Huang   Group: iGEM17_HZAU-China   (2017-10-20)
Revision as of 05:09, 25 October 2017 by Johnson Huang (Talk | contribs) (→‎Design Notes)


pMagFast


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 87
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The protein sequence is acquired from Kawano, F., Suzuki, H., Furuya, A., & Sato, M. (2015). Engineered pairs of distinct photoswitches for optogenetic control of cellular proteins. Nat Commun, 6, 6256. doi:10.1038/ncomms7256, and its DNA sequence is codon optimized for E. coli by IDT.

Source

This part is made from the VVD protein family derived from the fungus Neurospora crassa.

Reference Kawano, F., Suzuki, H., Furuya, A., & Sato, M. (2015). Engineered pairs of distinct photoswitches for optogenetic control of cellular proteins. Nat Commun, 6, 6256. doi:10.1038/ncomms7256

References