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Part:BBa_K2407107:Design

Designed by: Yan Zheng   Group: iGEM17_Tianjin   (2017-10-17)
Revision as of 04:57, 25 October 2017 by Tjuyoung (Talk | contribs) (→‎References)

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HO gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 270
    Illegal BglII site found at 954
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1290


Design Notes

Site-specific endonuclease; required for gene conversion at the MAT locus (homothallic switching) through the generation of a ds DNA break; expression restricted to mother cells in late G1 as controlled by Swi4p-Swi6p, Swi5p, and Ash1p.

Source

We use PCR to amplify the part from puc57-HO.

References

Stochastic expression and epigenetic memory at the yeast HO promoter. Zhang Q, et al. Proc Natl Acad Sci U S A, 2013 Aug 20. PMID 23836672 SWI/SNF binding to the HO promoter requires histone acetylation and stimulates TATA-binding protein recruitment. Mitra D, et al. Mol Cell Biol, 2006 Jun. PMID 16705163 A site-specific endonuclease essential for mating-type switching in Saccharomyces cerevisiae. Kostriken R, et al. Cell, 1983 Nov. PMID 6313222 Homothallic switching of yeast mating type cassettes is initiated by a double-stranded cut in the MAT locus. Strathern JN, et al. Cell, 1982 Nov. PMID 6297747 Nuclear export of Ho endonuclease of yeast via Msn5. Bakhrat A, et al. Curr Genet, 2008 Nov. PMID 18807043