Coding

Part:BBa_K2381000

Designed by: Wenqi Huang   Group: iGEM17_HZAU-China   (2017-10-20)
Revision as of 03:09, 25 October 2017 by Johnson Huang (Talk | contribs)


pMagFast

pMag, a light-induced dimerization protein derived from VVD protein family, mutates at I52R/M55R. It's corresponding protein, nMag also derived from the VVD protein, mutates at I52D/M55G. They can heterodimerize under blue light irritation. Their light sensing molecule is NAD which is normal in nearly all organisms. This part is pMag-Fast, whose dissociation rate is more rapid than the origin pMag. It's function is proved by parts BBa_K2381017

Reference

Kawano, F., Suzuki, H., Furuya, A., & Sato, M. (2015). Engineered pairs of distinct photoswitches for optogenetic control of cellular proteins. Nat Commun, 6, 6256. doi:10.1038/ncomms7256

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 87
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None