Translational_Unit

Part:BBa_K2505003

Designed by: Hazuki Hasegawa   Group: iGEM17_TokyoTech   (2017-10-16)
Revision as of 02:30, 25 October 2017 by TakumaY (Talk | contribs)

IVS-IRES-log1

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 225
    Illegal XhoI site found at 1275
    Illegal XhoI site found at 1287
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


The gene(log1) is derived from A.thaliana, optimized for H.sapiens codon in reference to the codon usage. LOG1 is an enzyme(putative lysine decarboxylase family) that necessary for a maturation of iP precursor.

Characterization

We tested the expression of LOG1 protein in human cell(EA.hy626) that necessary for maturation of iP precursor.

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Categories
Parameters
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