Coding

Part:BBa_K2429045:Design

Designed by: Nia Myrie   Group: iGEM17_MIT   (2017-10-24)
Revision as of 00:08, 25 October 2017 by Nmyrie (Talk | contribs) (→‎Design Notes)

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pTRE Ms2


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal EcoRI site found at 630
    Illegal XbaI site found at 30
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal EcoRI site found at 630
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal EcoRI site found at 630
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal EcoRI site found at 630
    Illegal XbaI site found at 30
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal EcoRI site found at 630
    Illegal XbaI site found at 30
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is human codon optimized

Source

The part's original source is the RNA bacteriophage. This part was obtained from another researcher who was kind enough to give us the sequence and physical plasmid containing the protein with the DDX6 domain sequence attached to the Ms2 sequence.


References