Coding

Part:BBa_K2267024:Design

Designed by: HE, BOTAO   Group: iGEM17_TUST_China   (2017-10-24)
Revision as of 22:13, 24 October 2017 by Registry (Talk | contribs)

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SpyTag-GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 866


Design Notes

A 6*His tag at the C end to facilitate purification. In order to increase the translation initiation rate, we also add an E.coil RBS.


Source

The SpyTag sequence comes from team Peking and sequence optimization is carried out by adding RBS and 6*His tag by PCR.

References