Coding
Part:BBa_K2267024:Design
Designed by: HE, BOTAO Group: iGEM17_TUST_China (2017-10-24)
SpyTag-GFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 866
Design Notes
A 6*His tag at the C end to facilitate purification. In order to increase the translation initiation rate, we also add an E.coil RBS.
Source
The SpyTag sequence comes from team Peking and sequence optimization is carried out by adding RBS and 6*His tag by PCR.