Reporter

Part:BBa_K2450301

Designed by: Zoe Ford   Group: iGEM17_Oxford   (2017-10-17)
Revision as of 20:30, 24 October 2017 by Tinyref (Talk | contribs) (→‎Usage and Biology)


pTet promoter coding eYFP

A pTet promoter and RBS encoding for YFP.

This part was designed to test the efficacy of a modified TetR (Tet repressor), BBa_K2450201. It is a medium strength pTet promotor.

This part will work as a reporter for a system relying on a change in the levels of TetR repression.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Usage and Biology

pTet is the promoter that can be bound by the Tet repressor, a dimer that is prevented from binding DNA by the presence of the antimicrobial tetracycline (ATC). TetR is a very tight-binding repressor, meaning that leakage is unlikely (in contrast to a system such as Lac).

We have demonstrated that our part works as participated, by plate reader experiments measuring the amount of YFP fluorescence produced over time in different conditions. The results are detailed below.

Functional Parameters

[edit]
Categories
Parameters
None