Reporter

Part:BBa_K2450301

Designed by: Zoe Ford   Group: iGEM17_Oxford   (2017-10-17)
Revision as of 19:08, 24 October 2017 by Tinyref (Talk | contribs)


pTet promoter coding eYFP

A pTet promoter and RBS encoding for YFP.

This part was designed to test the efficacy of a modified TetR (Tet repressor), BBa_K2450201. It is a medium strength pTet promotor.

This part will work as a reporter for a system relying on a change in the levels of TetR repression.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Usage and Biology

pTet is the promoter that can be bound by the Tet repressor, a dimer that is prevented from binding DNA by the presence of the antimicrobial tetracycline (ATC). TetR is a very tight-binding repressor, meaning that leakage is unlikely (in contrast to a system such as Lac).

Functional Parameters

[edit]
Categories
Parameters
None