DNA

Part:BBa_K2267000

Designed by: HE, BOTAO   Group: iGEM17_TUST_China   (2017-10-21)
Revision as of 14:13, 24 October 2017 by UndertoneTao (Talk | contribs)


a pH sensitive promoter Pasr

Explained

Asr promoter belongs to asr gene encoding acid-shock RNA. It is a pH-responsive promoter native to E.coli which induces transcription in acidic environment from pH 4.0 ~ 5.0 , and shows low activity at a neutral pH. We chose asr promoter as the base of pH regulation system.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 235
    Illegal SpeI site found at 2
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 235
    Illegal SpeI site found at 2
    Illegal NotI site found at 9
    Illegal NotI site found at 227
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 235
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 235
    Illegal SpeI site found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 235
    Illegal SpeI site found at 2
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None