Scar

Part:BBa_K2319004:Design

Designed by: Raj Magesh Gauthaman   Group: iGEM17_IISc-Bangalore   (2017-10-24)
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TAAG


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The extra nucleotide (G) is used to form the NheI restriction site when the T7 terminator (BBa_K731721) is added after it, which was then used in our subsequent assembly.


Source

This scar was added to our other part BBa_K2319000 (sfGFP-SpyCatcher under T7 expression system) using primers with overhangs.

References