Reporter

Part:BBa_K2276008

Designed by: Tianying Chen   Group: iGEM17_SCU_China   (2017-10-24)
Revision as of 10:27, 24 October 2017 by Registry (Talk | contribs)

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a composite of TetR repressible promoter、special designed RBS and the coding sequence of mRFP

The part was designed based on the Part:BBa_I13521,while the RBS binding site is modified. The RBS is special designed for strains whose last 9 nucleotides of the 16S rRNA is ACCTCCTTA.We want to generate a part which present a high translation rate, but this one did not meet our expectation.The promoter is TetR repressible promoter which is also used in the repressilator system. And the mRFP served as the reporter to help us measure the translation rate of the designed RBS.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 634
    Illegal AgeI site found at 746
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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