Device

Part:BBa_K2276009:Design

Designed by: Wei LIN   Group: iGEM17_SCU_China   (2017-10-24)
Revision as of 09:38, 24 October 2017 by Registry (Talk | contribs)

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pTetR-mOrange


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 843
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

If this part is transformed into a cell without TetR repressor, it will express mOrange constitutively. If this part is transformed into a cell expressing TetR repressor, it will be inhibited but can be derepessed by anhydrotetracycline or other tetracycline analogues.


Source

BBa_E2050, BBa_R0040

References