Coding
TCaiiAyhjH
Part:BBa_K2471003
Designed by: Armando Palacios Chaparro Group: iGEM17_Tec-Chihuahua (2017-10-24)
Revision as of 05:45, 24 October 2017 by Armando.Pach (Talk | contribs)
(T7 promoter + RBS) + (aiiA) + (RBS) + (yhjH) + (RBS) + (epsE) + (T1 terminator)
This BioBrick™ contains the necessary genetic circuitry to constitutively express the aiiA, yhjH and epsE genes, that when expressed, results in the production of the three enzymes of the same name. The combined effect of these will cause the cell to be unable to communicate (quorum quenching), move (motility inhibition) and form biofilm (decrease of ci-d-GMP).
A chassis with the T7 polymerase gene is needed for the successful expression of this BioBrick™.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2434
Illegal AgeI site found at 137
Illegal AgeI site found at 2184 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |